Marlina Adly
Andalas University, Indonesia
Title: Molecular Characterization of Vibrio parahaemolyticus Isolated From Different Raw Food Sources in Padang, West Sumatera, Indonesia
Biography
Biography: Marlina Adly
Abstract
Vibrio parahaemolyticus was identified from shrimp (Penaeus monodon), white shrimp (Penaeus merguensis), “kelong” shrimp (Penaeus indicus). The bacteria were recovered using chromagenic Vibrio agar. Isolated V. parahaemolyticus was further characterized by plasmid profiling. The virulence genes of the isolates (toxR, tdh, trh) that produce pathogenic V. parahaemolyticus were screened by PCR assay. The plasmid profiling analysis showed that out of 20 isolates, only 5 isolates contained plasmids. Specific PCR showed that 120 (100%) of the isolates carried toxR gene, 6 (9.7%) isolates possessed trh genes, only 1 (1.6%) have tdh gene.
